VZV ORF8 (UniProtKB – Q6QCP8) encodes a dUTPase, a metalloenzyme that catalyze the hydrolysis of dUTP to dUMP and pyrophosphate (Virology 234, 186–195 (1997). Its HSV1 orthologue is UL50. Kinetics of VZV ORF8 protein expression during lytic infection in vitro was assessed using western blot and our anti-VZV ORF8 antibody VZ 8.04 and ORF8 was detected between 8 and 10 hpi, typical for proteins involved in viral DNA replication at an early (E) phase of viral infection. Majority of this protein was located in the nucleus of the infected cell, but it was also found in the cytoplasm. Our anti-VZV8 antibody VZ 8.04 works in ELISA (on immunogen), IF (on VZV infected cells) and each LOT is validated for Western blot of VZV infected cell lysates, where it recognizes a protein of ~50 kDa in Western blot, which corresponds well to the predicted size of the protein encoded by the VZV ORF8 (described in J Virol. 2013 Jun;87(12):6943-54.).
Clone: VZ 8.04
Catalog No.: HR-VZV-02
Host Species: Mouse
Reactivity: Varicella zoster virus
Antigen/Immunogen: The immunogen consisted of full length ORF8 and was produced in E.coli
Tested Applications: ELISA; WB
Recommended Dilution: n/a
Varicella zoster virus
The immunogen consisted of full length ORF8 and was produced in E.coli
€200.00 – €700.00
|STORAGE||Long term -20 °C, short term +4 °C. Avoid freeze-thaw cycles.|
|LIGHT CHAIN TYPE||kappa|
|REFERENCES||Lenac et al., J Virol, 2013|
VZV ORF21 encodes a 115 kDa protein that is essential for viral replication in cell culture and is also expressed during, but is not required for establishment of latency in dorsal root and cranial nerve ganglia.
VZV ORF24 encodes a 30 kDa type 2 membrane protein that, together with the product of VZV ORF27, likely has a function in the transport (egress) of assembled viral capsids from the nuclei of VZV infected cells to the cytoplasm.
ORF48 encodes an alkaline nuclease (J Virol. 87: 11936-11938). ORF48 protein is homologous to herpes simplex type 1 (HSV-1) UL12, human cytomegalovirus (hCMV) UL98 and Epstein Barr virus (EBV) BGLF5.
VZV ORF33.5 encodes a scaffold protein involved in the assembly of the viral capsid. ORF33.5 has the capacity to mediate ORF40 translocation into the nucleus of VZV-infected cells and exhibits a nuclear localization phenotype.
The ORF53, which is predicted to be a tegument protein, consists of 331 amino acids and plays a critical role in cytoplasmic virus egress. ORF53 participates in the final step of tegumentation and envelope acquisition within the host cytoplasm.