VZV ORF8 (UniProtKB – Q6QCP8) encodes a dUTPase, a metalloenzyme that catalyze the hydrolysis of dUTP to dUMP and pyrophosphate (Virology 234, 186–195 (1997). Its HSV1 orthologue is UL50. Kinetics of VZV ORF8 protein expression during lytic infection in vitro was assessed using western blot and our anti-VZV ORF8 antibody VZ 8.04 and ORF8 was detected between 8 and 10 hpi, typical for proteins involved in viral DNA replication at an early (E) phase of viral infection. Majority of this protein was located in the nucleus of the infected cell, but it was also found in the cytoplasm. Our anti-VZV8 antibody VZ 8.04 works in ELISA (on immunogen), IF (on VZV infected cells) and each LOT is validated for Western blot of VZV infected cell lysates, where it recognizes a protein of ~50 kDa in Western blot, which corresponds well to the predicted size of the protein encoded by the VZV ORF8 (described in J Virol. 2013 Jun;87(12):6943-54.).
Clone: VZ 8.04
Catalog No.: HR-VZV-02
Host Species: Mouse
Reactivity: Varicella zoster virus
Antigen/Immunogen: The immunogen consisted of full length ORF8 and was produced in E.coli
Tested Applications: ELISA; WB
Recommended Dilution: n/a
Varicella zoster virus
The immunogen consisted of full length ORF8 and was produced in E.coli
€200.00 – €700.00
|STORAGE||Long term -20 °C, short term +4 °C. Avoid freeze-thaw cycles.|
|LIGHT CHAIN TYPE||kappa|
|REFERENCES||Lenac et al., J Virol, 2013|
The ORF50 gene of the varicella-zoster virus (VZV) encodes glycoprotein M which is conserved among all herpesviruses. VZV gM is predicted to be an eight-transmembrane envelope glycoprotein modified with a complex N-linked oligosaccharide.
VZV ORF18 (UniProtKB – Q6QCN7) encodes a small subunit of the viral ribonucleotide reductase, which together with the large subunit, encoded by the VZV ORF19, forms the ribonucleotide reductase holoenzyme complex (RNR)
ORF31 is one of five glycoprotein ORFs in VZV genome. It encodes for gB, a type I membrane protein containing 868 aa which in mature form exists as a highly glycosylated disulfide-linked heterodimer (Annu Rev Microbiol 1990. 44: 59-80).
VZV ORF23 encodes a 24 kDa small capsid surface protein that localizes primarily to cell nuclei during infection and is not essential for viral replication in cell culture, even though its absence disrupts the capsid assembly.