VZV ORF8 (UniProtKB – Q6QCP8) encodes a dUTPase, a metalloenzyme that catalyze the hydrolysis of dUTP to dUMP and pyrophosphate (Virology 234, 186–195 (1997). Its HSV1 orthologue is UL50. Kinetics of VZV ORF8 protein expression during lytic infection in vitro was assessed using western blot and our anti-VZV ORF8 antibody VZ 8.04 and ORF8 was detected between 8 and 10 hpi, typical for proteins involved in viral DNA replication at an early (E) phase of viral infection. Majority of this protein was located in the nucleus of the infected cell, but it was also found in the cytoplasm. Our anti-VZV8 antibody VZ 8.04 works in ELISA (on immunogen), IF (on VZV infected cells) and each LOT is validated for Western blot of VZV infected cell lysates, where it recognizes a protein of ~50 kDa in Western blot, which corresponds well to the predicted size of the protein encoded by the VZV ORF8 (described in J Virol. 2013 Jun;87(12):6943-54.).