VZV ORF21 encodes a 115 kDa protein that is essential for viral replication in cell culture and is also expressed during, but is not required for establishment of latency in dorsal root and cranial nerve ganglia. During infection, VZV ORF21 protein localizes to both nuclei and cytoplasm of infected cells and, together with proteins encoded by VZV ORFs 20, 21, 23, 33, 40 and 41, forms the icosahedral nucleocapsid core of VZV virions. In addition, protease treatment and detergent solubilization experiments of VZV virions suggest that VZV ORF21 encoded protein is not associated with tegument of VZV particles. Our anti-VZV ORF21 antibody VZ 21.3.05 works in ELISA (on immunogen) and IF (on VZV infected cells), and each LOT is validated for Western blot (of VZV infected cell lysates), where it specifically recognizes a protein species of ~116 kDa, which corresponds well to the predicted size of ~115 kDa protein encoded by the VZV ORF 21 (described in J Virol. 2013 Jun;87(12):6943-54.).
Clone: VZ 21.3.05
Catalog No.: HR-VZV-11
Host Species: Mouse
Reactivity: Varicella zoster virus
Antigen/Immunogen: The immunogen consisted of ORF21 C terminal domain (amino acids 667-1039) and was produced in E.coli
Tested Applications: ELISA; WB
Recommended Dilution: n/a
Varicella zoster virus
The immunogen consisted of ORF21 C terminal domain (amino acids 667-1039) and was produced in E.coli
€200.00 – €700.00
|STORAGE||Long term -20 °C, short term +4 °C. Avoid freeze-thaw cycles.|
|LIGHT CHAIN TYPE||kappa|
|REFERENCES||Lenac et al., J Virol, 2013|
VZV ORF19 encodes a large, N-terminal subunit 1 of the viral ribonucleotide reductase, which together with the small subunit, encoded by the VZV ORF 18, forms the ribonucleotide reductase holoenzyme complex (RNR).
VZV ORF18 (UniProtKB – Q6QCN7) encodes a small subunit of the viral ribonucleotide reductase, which together with the large subunit, encoded by the VZV ORF19, forms the ribonucleotide reductase holoenzyme complex (RNR)
VZV ORF27 encodes a nuclear egress lamina protein, that together with the nuclear egress membrane protein encoded by the product of VZV ORF27, most likely plays a role in the egress of assembled viral capsids from the nuclei of VZV infected cells to the cytoplasm.