Open reading frame 68 (ORF68) of Varicella zoster encodes for essential, most immunogenic and most abundant glycoprotein E (gE). VZV gE is a typical type 1 transmembrane protein containing both N-linked and O-linked glycans, sialylation, sulfation and Ser/Thr and Tyr phosphorylation and fully modified has 98-kDa (Journal of Virology (1997) 71, 110-9). It forms a complex with gI (ORF67) during viral infection. Mature VZV gE/gI complex can be found predominantly on the cell membrane of infected cells in culture and in vivo and it is subsequently internalized through use of the respective internalization signals (Journal of Virology (1998) 72, 1542-51). This complex shows high similarity with FcgRII and binds human (rabbit) IgG Fc fragment (Journal of Virology (1992) 66, 3643–3651, Virology (1190) 178, 263-72). VZV gE-gB coexpression enhanced the fusogenic properties of gB and can substitute gL to facilitate trafficking of gH to the cell surface (Journal of Virology (2001) 75, 9483–9492). Our anti-VZV ORF68 antibody VZ 68.03 works in ELISA (on immunogen), IF (on VZV infected cells) and each LOT is validated for Western blot (of VZV infected cell lysates, described in described in J Virol. 2013 Jun;87(12):6943-54.).
Clone: VZ 68.03
Catalog No.: HR-VZV-27
Host Species: Mouse
Reactivity: Varicella zoster virus
Antigen/Immunogen: The immunogen consisted of full length ORF68 and was produced in E.coli
Tested Applications: ELISA; WB
Recommended Dilution: n/a
Varicella zoster virus
The immunogen consisted of full length ORF68 and was produced in E.coli
€200.00 – €700.00
|STORAGE||Long term -20 °C, short term +4 °C. Avoid freeze-thaw cycles.|
|LIGHT CHAIN TYPE||kappa|
|REFERENCES||Lenac et al., J Virol, 2013|
VZV ORF21 encodes a 115 kDa protein that is essential for viral replication in cell culture and is also expressed during, but is not required for establishment of latency in dorsal root and cranial nerve ganglia.
The ORF53, which is predicted to be a tegument protein, consists of 331 amino acids and plays a critical role in cytoplasmic virus egress. ORF53 participates in the final step of tegumentation and envelope acquisition within the host cytoplasm.
The ORF50 gene of the varicella-zoster virus (VZV) encodes glycoprotein M which is conserved among all herpesviruses. VZV gM is predicted to be an eight-transmembrane envelope glycoprotein modified with a complex N-linked oligosaccharide.
ORF48 encodes an alkaline nuclease (J Virol. 87: 11936-11938). ORF48 protein is homologous to herpes simplex type 1 (HSV-1) UL12, human cytomegalovirus (hCMV) UL98 and Epstein Barr virus (EBV) BGLF5.
VZV ORF27 encodes a nuclear egress lamina protein, that together with the nuclear egress membrane protein encoded by the product of VZV ORF27, most likely plays a role in the egress of assembled viral capsids from the nuclei of VZV infected cells to the cytoplasm.