Open reading frame 68 (ORF68) of Varicella zoster encodes for essential, most immunogenic and most abundant glycoprotein E (gE). VZV gE is a typical type 1 transmembrane protein containing both N-linked and O-linked glycans, sialylation, sulfation and Ser/Thr and Tyr phosphorylation and fully modified has 98-kDa (Journal of Virology (1997) 71, 110-9). It forms a complex with gI (ORF67) during viral infection. Mature VZV gE/gI complex can be found predominantly on the cell membrane of infected cells in culture and in vivo and it is subsequently internalized through use of the respective internalization signals (Journal of Virology (1998) 72, 1542-51). This complex shows high similarity with FcgRII and binds human (rabbit) IgG Fc fragment (Journal of Virology (1992) 66, 3643–3651, Virology (1190) 178, 263-72). VZV gE-gB coexpression enhanced the fusogenic properties of gB and can substitute gL to facilitate trafficking of gH to the cell surface (Journal of Virology (2001) 75, 9483–9492). Our anti-VZV ORF68 antibody VZ 68.03 works in ELISA (on immunogen), IF (on VZV infected cells) and each LOT is validated for Western blot (of VZV infected cell lysates, described in described in J Virol. 2013 Jun;87(12):6943-54.).
Clone: VZ 68.03
Catalog No.: HR-VZV-27
Host Species: Mouse
Reactivity: Varicella zoster virus
Antigen/Immunogen: The immunogen consisted of full length ORF68 and was produced in E.coli
Tested Applications: ELISA; WB
Recommended Dilution: n/a
Varicella zoster virus
The immunogen consisted of full length ORF68 and was produced in E.coli
€200.00 – €700.00
|STORAGE||Long term -20 °C, short term +4 °C. Avoid freeze-thaw cycles.|
|LIGHT CHAIN TYPE||kappa|
|REFERENCES||Lenac et al., J Virol, 2013|
VZV ORF23 encodes a 24 kDa small capsid surface protein that localizes primarily to cell nuclei during infection and is not essential for viral replication in cell culture, even though its absence disrupts the capsid assembly.
Immediate-early 62 protein (IE62) is a homolog of herpes simplex type 1 (HSV-1) ICP4. These two proteins have conserved DNA bidnding domain,while not activation domain (J Virol 1993. 67: 4246-4251).
VZV ORF18 (UniProtKB – Q6QCN7) encodes a small subunit of the viral ribonucleotide reductase, which together with the large subunit, encoded by the VZV ORF19, forms the ribonucleotide reductase holoenzyme complex (RNR)
VZV ORF24 encodes a 30 kDa type 2 membrane protein that, together with the product of VZV ORF27, likely has a function in the transport (egress) of assembled viral capsids from the nuclei of VZV infected cells to the cytoplasm.