Open reading frame 68 (ORF68) of Varicella zoster encodes for essential, most immunogenic and most abundant glycoprotein E (gE). VZV gE is a typical type 1 transmembrane protein containing both N-linked and O-linked glycans, sialylation, sulfation and Ser/Thr and Tyr phosphorylation and fully modified has 98-kDa (Journal of Virology (1997) 71, 110-9). It forms a complex with gI (ORF67) during viral infection. Mature VZV gE/gI complex can be found predominantly on the cell membrane of infected cells in culture and in vivo and it is subsequently internalized through use of the respective internalization signals (Journal of Virology (1998) 72, 1542-51). This complex shows high similarity with FcgRII and binds human (rabbit) IgG Fc fragment (Journal of Virology (1992) 66, 3643–3651, Virology (1190) 178, 263-72). VZV gE-gB coexpression enhanced the fusogenic properties of gB and can substitute gL to facilitate trafficking of gH to the cell surface (Journal of Virology (2001) 75, 9483–9492). Our anti-VZV ORF68 antibody VZ 68.03 works in ELISA (on immunogen), IF (on VZV infected cells) and each LOT is validated for Western blot (of VZV infected cell lysates, described in described in J Virol. 2013 Jun;87(12):6943-54.).
Clone: VZ 68.03
Catalog No.: HR-VZV-27
Host Species: Mouse
Reactivity: Varicella zoster virus
Antigen/Immunogen: The immunogen consisted of full length ORF68 and was produced in E.coli
Tested Applications: ELISA; WB
Recommended Dilution: n/a
Varicella zoster virus
The immunogen consisted of full length ORF68 and was produced in E.coli
€200.00 – €700.00
|STORAGE||Long term -20 °C, short term +4 °C. Avoid freeze-thaw cycles.|
|LIGHT CHAIN TYPE||kappa|
|REFERENCES||Lenac et al., J Virol, 2013|
The ORF53, which is predicted to be a tegument protein, consists of 331 amino acids and plays a critical role in cytoplasmic virus egress. ORF53 participates in the final step of tegumentation and envelope acquisition within the host cytoplasm.
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