Open reading frame 14 (ORF14) of Varicella zoster encodes for late glycoprotein C (gC). VZV gC is 105-kDa highly glycosylated protein with almost one third of its molecular weight consisting of oligosaccharide chains on four predicted N-linked glycosylation sites and 25 O-linked mucin type sites (Journal of Virology (1990) 64, 4540-8, Virology (2008) 382, 171-81). Unlike other major glycoproteins in herpesviruses, it shares only 30% homology with HSV-1 gC (Reviews of infectious diseases (1991) 13, pp. S960–S963). It shows delayed transcription and protein expression when compared with other major VZV glycoproteins (gE, gI, gH and gB) as well compared with HSV gC in infected cells (Virology (2008) 382, 171-81, Current Topics in Microbiology and Immunology (2010) 342, 113-28. VZV gC expression is reduced in VZV-infected neurons on both transcriptional and protein level (Journal of Virology (2013) 87, 9643–9648 6). Our anti VZV ORF14 antibody VZ 14.12 shows that the VZV gC is located predominantly in the Golgi. Our anti-VZV ORF14 antibody VZ 14.12 works in ELISA (on immunogen), IF (on VZV infected cells) and each LOT is validated for Western blot (of VZV infected cell lysates, described in J Virol. 2013 Jun;87(12):6943-54.).
Clone: VZ 14.12
Catalog No.: HR-VZV-09
Host Species: Mouse
Reactivity: Varicella zoster virus
Antigen/Immunogen: The immunogen consisted of full length ORF14 and was produced in E.coli
Tested Applications: ELISA; IF; WB
Varicella zoster virus
The immunogen consisted of full length ORF14 and was produced in E.coli
ELISA; IF; WB
€120.00 – €500.00
|STORAGE||Long term -20 °C, short term +4 °C. Avoid freeze-thaw cycles.|
|LIGHT CHAIN TYPE||kappa|
|REFERENCES||Lenac et al., J Virol, 2013|
VZV ORF19 encodes a large, N-terminal subunit 1 of the viral ribonucleotide reductase, which together with the small subunit, encoded by the VZV ORF 18, forms the ribonucleotide reductase holoenzyme complex (RNR).
VZV ORF18 (UniProtKB – Q6QCN7) encodes a small subunit of the viral ribonucleotide reductase, which together with the large subunit, encoded by the VZV ORF19, forms the ribonucleotide reductase holoenzyme complex (RNR)
VZV ORF27 encodes a nuclear egress lamina protein, that together with the nuclear egress membrane protein encoded by the product of VZV ORF27, most likely plays a role in the egress of assembled viral capsids from the nuclei of VZV infected cells to the cytoplasm.
VZV ORF23 encodes a 24 kDa small capsid surface protein that localizes primarily to cell nuclei during infection and is not essential for viral replication in cell culture, even though its absence disrupts the capsid assembly.