The ORF22 encodes for the inner tegument protein UL36 that plays multiple roles in the viral cycle. During viral entry it remains associated with the capsid, participates in the capsid transport toward the host nucleus and by interacting with the nuclear pore complex (NPC) faciliate release of viral DNA from the nucleocapsids. Within the host nucleus, acts as a deneddylase and promotes the degradation of nuclear CRLs (cullin-RING ubiquitin ligases) and thereby stabilizes nuclear CRL substrates, while cytoplasmic CRLs remain unaffected. These modifications prevent host cell cycle S-phase progression and create a favourable environment allowing efficient viral genome replication. During viral maturation plays a linker role in association of the outer viral tegument to the capsids together with the inner tegument protein. Our anti-VZV ORF 22 antibody VZ 22.3.10 (described in J Virol. 2013 Jun;87(12):6943-54.) works in ELISA (on immunogen), IF (on VZV infected cells) and each LOT is validated for Western blot (of VZV infected cell lysates) where it specifically recognizes a protein species of ~306 kDa in lysates obtained from VZV infected cells.
Clone: VZ 22.3.10
Catalog No.: HR-VZV-26
Host Species: Mouse
Reactivity: Varicella zoster virus
Antigen/Immunogen: The immunogen consisted ofa fragment of ORF22 protein and was produced in E.coli
Tested Applications: ELISA; WB
Recommended Dilution: n/a
Varicella zoster virus
The immunogen consisted ofa fragment of ORF22 protein and was produced in E.coli
€200.00 – €700.00
|STORAGE||Long term -20 °C, short term +4 °C. Avoid freeze-thaw cycles.|
|LIGHT CHAIN TYPE||kappa|
|REFERENCES||Lenac et al., J Virol, 2013|
Immediate-early 62 protein (IE62) is a homolog of herpes simplex type 1 (HSV-1) ICP4. These two proteins have conserved DNA bidnding domain,while not activation domain (J Virol 1993. 67: 4246-4251).
The ORF53, which is predicted to be a tegument protein, consists of 331 amino acids and plays a critical role in cytoplasmic virus egress. ORF53 participates in the final step of tegumentation and envelope acquisition within the host cytoplasm.
VZV ORF4 encodes an IE4 protein that acts as transactivator of several immediate early, early and late VZV genes. Dimerization of IE4 is required for its transactivation function.
VZV ORF33.5 encodes a scaffold protein involved in the assembly of the viral capsid. ORF33.5 has the capacity to mediate ORF40 translocation into the nucleus of VZV-infected cells and exhibits a nuclear localization phenotype.