The ORF22 encodes for the inner tegument protein UL36 that plays multiple roles in the viral cycle. During viral entry it remains associated with the capsid, participates in the capsid transport toward the host nucleus and by interacting with the nuclear pore complex (NPC) faciliate release of viral DNA from the nucleocapsids. Within the host nucleus, acts as a deneddylase and promotes the degradation of nuclear CRLs (cullin-RING ubiquitin ligases) and thereby stabilizes nuclear CRL substrates, while cytoplasmic CRLs remain unaffected. These modifications prevent host cell cycle S-phase progression and create a favourable environment allowing efficient viral genome replication. During viral maturation plays a linker role in association of the outer viral tegument to the capsids together with the inner tegument protein. Our anti-VZV ORF 22 antibody VZ 22.3.10 (described in J Virol. 2013 Jun;87(12):6943-54.) works in ELISA (on immunogen), IF (on VZV infected cells) and each LOT is validated for Western blot (of VZV infected cell lysates) where it specifically recognizes a protein species of ~306 kDa in lysates obtained from VZV infected cells.
Clone: VZ 22.3.10
Catalog No.: HR-VZV-26
Host Species: Mouse
Reactivity: Varicella zoster virus
Antigen/Immunogen: The immunogen consisted ofa fragment of ORF22 protein and was produced in E.coli
Tested Applications: ELISA; WB
Recommended Dilution: n/a
Varicella zoster virus
The immunogen consisted ofa fragment of ORF22 protein and was produced in E.coli
€200.00 – €700.00
|STORAGE||Long term -20 °C, short term +4 °C. Avoid freeze-thaw cycles.|
|LIGHT CHAIN TYPE||kappa|
|REFERENCES||Lenac et al., J Virol, 2013|
VZV ORF18 (UniProtKB – Q6QCN7) encodes a small subunit of the viral ribonucleotide reductase, which together with the large subunit, encoded by the VZV ORF19, forms the ribonucleotide reductase holoenzyme complex (RNR)
VZV ORF27 encodes a nuclear egress lamina protein, that together with the nuclear egress membrane protein encoded by the product of VZV ORF27, most likely plays a role in the egress of assembled viral capsids from the nuclei of VZV infected cells to the cytoplasm.
VZV ORF21 encodes a 115 kDa protein that is essential for viral replication in cell culture and is also expressed during, but is not required for establishment of latency in dorsal root and cranial nerve ganglia.