VZV ORF57 encodes a poorly characterized protein of predicted 8 kDa molecular mass. Initial immunoprecipitation experiments using antibodies to ORF57 protein revealed a 6 kDa protein in the cytoplasm of infected cells that was dispensable for viral replication in cell culture. Later, we have observed a membrane associated localization of ORF57 as well as its localization in the Golgi-network. Based on performed analyses, it has presumed that the product of VZV ORF57 might play a role in virion morphogenesis. Our anti-VZV ORF57 antibody VZ 57.10 works in ELISA (on immunogen) and IF (on VZV infected cells) and each LOT is validated for Western blot (of VZV infected cell lysates) where it specifically recognizes a protein species of ~12 kDa in lysates obtained from VZV infected cells (described in J Virol. 2013 Jun;87(12):6943-54.).
Clone: VZ 57.10
Catalog No.: HR-VZV-18
Host Species: Mouse
Reactivity: Varicella zoster virus
Antigen/Immunogen: The immunogen consisted of full length ORF57 and was produced in E.coli
Tested Applications: ELISA; WB
Varicella zoster virus
The immunogen consisted of full length ORF57 and was produced in E.coli
€120.00 – €500.00
|STORAGE||Long term -20 °C, short term +4 °C. Avoid freeze-thaw cycles.|
|LIGHT CHAIN TYPE||kappa|
|REFERENCES||Lenac et al., J Virol, 2013|
VZV ORF21 encodes a 115 kDa protein that is essential for viral replication in cell culture and is also expressed during, but is not required for establishment of latency in dorsal root and cranial nerve ganglia.
VZV ORF23 encodes a 24 kDa small capsid surface protein that localizes primarily to cell nuclei during infection and is not essential for viral replication in cell culture, even though its absence disrupts the capsid assembly.
VZV ORF33.5 encodes a scaffold protein involved in the assembly of the viral capsid. ORF33.5 has the capacity to mediate ORF40 translocation into the nucleus of VZV-infected cells and exhibits a nuclear localization phenotype.
ORF48 encodes an alkaline nuclease (J Virol. 87: 11936-11938). ORF48 protein is homologous to herpes simplex type 1 (HSV-1) UL12, human cytomegalovirus (hCMV) UL98 and Epstein Barr virus (EBV) BGLF5.