VZV ORF57 encodes a poorly characterized protein of predicted 8 kDa molecular mass. Initial immunoprecipitation experiments using antibodies to ORF57 protein revealed a 6 kDa protein in the cytoplasm of infected cells that was dispensable for viral replication in cell culture. Later, we have observed a membrane associated localization of ORF57 as well as its localization in the Golgi-network. Based on performed analyses, it has presumed that the product of VZV ORF57 might play a role in virion morphogenesis. Our anti-VZV ORF57 antibody VZ 57.10 works in ELISA (on immunogen) and IF (on VZV infected cells) and each LOT is validated for Western blot (of VZV infected cell lysates) where it specifically recognizes a protein species of ~12 kDa in lysates obtained from VZV infected cells (described in J Virol. 2013 Jun;87(12):6943-54.).
Clone: VZ 57.10
Catalog No.: HR-VZV-18
Host Species: Mouse
Reactivity: Varicella zoster virus
Antigen/Immunogen: The immunogen consisted of full length ORF57 and was produced in E.coli
Tested Applications: ELISA; WB
Recommended Dilution: n/a
Varicella zoster virus
The immunogen consisted of full length ORF57 and was produced in E.coli
€200.00 – €700.00
|STORAGE||Long term -20 °C, short term +4 °C. Avoid freeze-thaw cycles.|
|LIGHT CHAIN TYPE||kappa|
|REFERENCES||Lenac et al., J Virol, 2013|
ORF48 encodes an alkaline nuclease (J Virol. 87: 11936-11938). ORF48 protein is homologous to herpes simplex type 1 (HSV-1) UL12, human cytomegalovirus (hCMV) UL98 and Epstein Barr virus (EBV) BGLF5.
ORF31 is one of five glycoprotein ORFs in VZV genome. It encodes for gB, a type I membrane protein containing 868 aa which in mature form exists as a highly glycosylated disulfide-linked heterodimer (Annu Rev Microbiol 1990. 44: 59-80).
VZV ORF27 encodes a nuclear egress lamina protein, that together with the nuclear egress membrane protein encoded by the product of VZV ORF27, most likely plays a role in the egress of assembled viral capsids from the nuclei of VZV infected cells to the cytoplasm.