VZV ORF18 (UniProtKB – Q6QCN7) encodes a small subunit of the viral ribonucleotide reductase, which together with the large subunit, encoded by the VZV ORF19, forms the ribonucleotide reductase holoenzyme complex (RNR) (Curr. Top. Microbiol. Immunol. 342, 1–14 (2010)). RNR acts as a key enzyme that catalyzes the reductive synthesis of deoxyribonucleotides from corresponding ribonucleotides, thus providing precursors for viral DNA replication in non-dividing cells, as well as enabling reactivation of the virus in latently infected cells. VZV ORF18 is an orthologue of HSV1 protein UL40. Kinetics of VZV ORF8 protein expression during lytic infection in vitro was assessed using western blot and our anti-VZV18 antibody VZ 18C.16 and ORF18 were detected between 8 and 10 hpi, typical for proteins involved in viral DNA replication at an early (E) phase of viral infection. Our anti-VZV18 antibody VZ 18C.16 works in ELISA (on immunogen) and each LOT is validated for Western blot of VZV infected cell lysates, where it recognizes a protein of ~36 kDa, which corresponds well to the predicted protein size (described in J Virol. 2013 Jun;87(12):6943-54.).
Clone: VZ 18C.16
Catalog No.: HR-VZV-05
Host Species: Mouse
Reactivity: Varicella zoster virus
Antigen/Immunogen: The immunogen consisted of ORF18 C terminal domain (amino acids 168-306) and was produced in E.coli
Tested Applications: ELISA; WB
Recommended Dilution: n/a
Varicella zoster virus
The immunogen consisted of ORF18 C terminal domain (amino acids 168-306) and was produced in E.coli
€200.00 – €700.00
|STORAGE||Long term -20 °C, short term +4 °C. Avoid freeze-thaw cycles.|
|LIGHT CHAIN TYPE||kappa|
|REFERENCES||Lenac et al., J Virol, 2013|
ORF31 is one of five glycoprotein ORFs in VZV genome. It encodes for gB, a type I membrane protein containing 868 aa which in mature form exists as a highly glycosylated disulfide-linked heterodimer (Annu Rev Microbiol 1990. 44: 59-80).
Immediate-early 62 protein (IE62) is a homolog of herpes simplex type 1 (HSV-1) ICP4. These two proteins have conserved DNA bidnding domain,while not activation domain (J Virol 1993. 67: 4246-4251).
The ORF50 gene of the varicella-zoster virus (VZV) encodes glycoprotein M which is conserved among all herpesviruses. VZV gM is predicted to be an eight-transmembrane envelope glycoprotein modified with a complex N-linked oligosaccharide.