VZV ORF18 (UniProtKB – Q6QCN7) encodes a small subunit of the viral ribonucleotide reductase, which together with the large subunit, encoded by the VZV ORF19, forms the ribonucleotide reductase holoenzyme complex (RNR) (Curr. Top. Microbiol. Immunol. 342, 1–14 (2010)). RNR acts as a key enzyme that catalyzes the reductive synthesis of deoxyribonucleotides from corresponding ribonucleotides, thus providing precursors for viral DNA replication in non-dividing cells, as well as enabling reactivation of the virus in latently infected cells. VZV ORF18 is an orthologue of HSV1 protein UL40. Kinetics of VZV ORF8 protein expression during lytic infection in vitro was assessed using western blot and our anti-VZV18 antibody VZ 18C.16 and ORF18 were detected between 8 and 10 hpi, typical for proteins involved in viral DNA replication at an early (E) phase of viral infection. Our anti-VZV18 antibody VZ 18C.16 works in ELISA (on immunogen) and each LOT is validated for Western blot of VZV infected cell lysates, where it recognizes a protein of ~36 kDa, which corresponds well to the predicted protein size (described in J Virol. 2013 Jun;87(12):6943-54.).
Clone: VZ 18C.16
Catalog No.: HR-VZV-05
Host Species: Mouse
Reactivity: Varicella zoster virus
Antigen/Immunogen: The immunogen consisted of ORF18 C terminal domain (amino acids 168-306) and was produced in E.coli
Tested Applications: ELISA; WB
Varicella zoster virus
The immunogen consisted of ORF18 C terminal domain (amino acids 168-306) and was produced in E.coli
€120.00 – €500.00
|STORAGE||Long term -20 °C, short term +4 °C. Avoid freeze-thaw cycles.|
|LIGHT CHAIN TYPE||kappa|
|REFERENCES||Lenac et al., J Virol, 2013|
Immediate-early 62 protein (IE62) is a homolog of herpes simplex type 1 (HSV-1) ICP4. These two proteins have conserved DNA bidnding domain,while not activation domain (J Virol 1993. 67: 4246-4251).
VZV ORF27 encodes a nuclear egress lamina protein, that together with the nuclear egress membrane protein encoded by the product of VZV ORF27, most likely plays a role in the egress of assembled viral capsids from the nuclei of VZV infected cells to the cytoplasm.
VZV ORF19 encodes a large, N-terminal subunit 1 of the viral ribonucleotide reductase, which together with the small subunit, encoded by the VZV ORF 18, forms the ribonucleotide reductase holoenzyme complex (RNR).
VZV ORF4 encodes an IE4 protein that acts as transactivator of several immediate early, early and late VZV genes. Dimerization of IE4 is required for its transactivation function.