VZV ORF13 (UniProtKB – Q6QCP2) encodes a thymidylate synthetase (TS) which catalyzes the reductive methylation of deoxyuridylate to thymidylate. It is one of VZV’s five unique genes (ORF1, ORF2, ORF13, ORF32, and ORF57) not present in herpes simplex virus 1 (HSV-1). Our anti-VZV13 antibody VZ 13.15 detected ORF13 predominantly in the center of syncytia and the Golgi network. The interaction of ORF13 with ORF6, a component of the DNA helicase-primase complex, suggests a regulatory interaction between precursor synthesis and DNA encapsidation and implies the partial localization of ORF13 to the nucleus. This observation was confirmed by confocal analysis. Our anti-VZV13 antibody VZ 13.15 works in ELISA (on immunogen), IF (on VZV infected cells) and is validated for Western blot of VZV infected cell lysates, where it recognizes a protein of ~35 kDa, which corresponds well to the predicted size of the protein encoded by the VZV ORF 13 (described in J Virol. 2013 Jun;87(12):6943-54.).
Clone: VZ 13.15
Catalog No.: HR-VZV-04
Host Species: Mouse
Reactivity: Varicella zoster virus
Antigen/Immunogen: The immunogen consisted of full length ORF13 and was produced in E.coli
Tested Applications: ELISA; WB
Recommended Dilution: n/a
Varicella zoster virus
The immunogen consisted of full length ORF13 and was produced in E.coli
€200.00 – €700.00
|STORAGE||Long term -20 °C, short term +4 °C. Avoid freeze-thaw cycles.|
|LIGHT CHAIN TYPE||kappa|
|REFERENCES||Lenac et al., J Virol, 2013|
VZV ORF23 encodes a 24 kDa small capsid surface protein that localizes primarily to cell nuclei during infection and is not essential for viral replication in cell culture, even though its absence disrupts the capsid assembly.
ORF31 is one of five glycoprotein ORFs in VZV genome. It encodes for gB, a type I membrane protein containing 868 aa which in mature form exists as a highly glycosylated disulfide-linked heterodimer (Annu Rev Microbiol 1990. 44: 59-80).
VZV ORF18 (UniProtKB – Q6QCN7) encodes a small subunit of the viral ribonucleotide reductase, which together with the large subunit, encoded by the VZV ORF19, forms the ribonucleotide reductase holoenzyme complex (RNR)