VZV ORF13 (UniProtKB – Q6QCP2) encodes a thymidylate synthetase (TS) which catalyzes the reductive methylation of deoxyuridylate to thymidylate. It is one of VZV’s five unique genes (ORF1, ORF2, ORF13, ORF32, and ORF57) not present in herpes simplex virus 1 (HSV-1). Our anti-VZV13 antibody VZ 13.15 detected ORF13 predominantly in the center of syncytia and the Golgi network. The interaction of ORF13 with ORF6, a component of the DNA helicase-primase complex, suggests a regulatory interaction between precursor synthesis and DNA encapsidation and implies the partial localization of ORF13 to the nucleus. This observation was confirmed by confocal analysis. Our anti-VZV13 antibody VZ 13.15 works in ELISA (on immunogen), IF (on VZV infected cells) and is validated for Western blot of VZV infected cell lysates, where it recognizes a protein of ~35 kDa, which corresponds well to the predicted size of the protein encoded by the VZV ORF 13 (described in J Virol. 2013 Jun;87(12):6943-54.).
Clone: VZ 13.15
Catalog No.: HR-VZV-04
Host Species: Mouse
Reactivity: Varicella zoster virus
Antigen/Immunogen: The immunogen consisted of full length ORF13 and was produced in E.coli
Tested Applications: ELISA; WB
Recommended Dilution: n/a
Varicella zoster virus
The immunogen consisted of full length ORF13 and was produced in E.coli
€200.00 – €700.00
|STORAGE||Long term -20 °C, short term +4 °C. Avoid freeze-thaw cycles.|
|LIGHT CHAIN TYPE||kappa|
|REFERENCES||Lenac et al., J Virol, 2013|
ORF48 encodes an alkaline nuclease (J Virol. 87: 11936-11938). ORF48 protein is homologous to herpes simplex type 1 (HSV-1) UL12, human cytomegalovirus (hCMV) UL98 and Epstein Barr virus (EBV) BGLF5.
The ORF53, which is predicted to be a tegument protein, consists of 331 amino acids and plays a critical role in cytoplasmic virus egress. ORF53 participates in the final step of tegumentation and envelope acquisition within the host cytoplasm.
VZV ORF24 encodes a 30 kDa type 2 membrane protein that, together with the product of VZV ORF27, likely has a function in the transport (egress) of assembled viral capsids from the nuclei of VZV infected cells to the cytoplasm.
VZV ORF21 encodes a 115 kDa protein that is essential for viral replication in cell culture and is also expressed during, but is not required for establishment of latency in dorsal root and cranial nerve ganglia.