The envelope glycoprotein I of VZV, gI (UniProtKB – Q775H9) is an ortholog of HSV-1 protein US7. Mallory et al showed that the expression of ORF67 gene product is required for efficient VZV replication (J Infect Dis (1998) 178 (Supplement_1): S22-S26.). Oliver et al showed that intact gI structure required for gE/gI heterodimer formation is required for effective viral spread in human skin (J Virol. 2011 May; 85(9): 4095–4110). Different monoclonal antibodies to VZV ORF67 are available. The ORF67 has calculated molecular weight of 40 kDa and our monoclonal antibody VZ 67C.02 confirmed a significantly bigger glycoprotein at 50-70 kDa in western blot. Our VZ 67C.02 antibody confirmed that VZV ORF67 protein is localized to the Golgi and other membranes. Our anti-VZV ORF67 antibody VZ 67C.02 works in ELISA (on immunogen), d IF (on VZV infected cells) and each LOT is validated for Western blot (of VZV infected cell lysates, described in J Virol. 2013 Jun;87(12):6943-54.).
Clone: VZ 67C.02
Catalog No.: HR-VZV-32
Host Species: Mouse
Reactivity: Varicella zoster virus
Antigen/Immunogen: The immunogen consisted of ORF67 C terminal domain (amino acids 294-354) and was produced in E.coli
Tested Applications: ELISA; WB
Varicella zoster virus
The immunogen consisted of ORF67 C terminal domain (amino acids 294-354) and was produced in E.coli
€120.00 – €500.00
|STORAGE||Long term -20 °C, short term +4 °C. Avoid freeze-thaw cycles.|
|LIGHT CHAIN TYPE||kappa|
|REFERENCES||Lenac et al., J Virol, 2013|
The ORF50 gene of the varicella-zoster virus (VZV) encodes glycoprotein M which is conserved among all herpesviruses. VZV gM is predicted to be an eight-transmembrane envelope glycoprotein modified with a complex N-linked oligosaccharide.
Open reading frame 37 (ORF37) of Varicella zoster encodes for envelope glycoprotein H (gH). VZV gH is a 118kDa type 1 transmembrane glycoprotein highly conserved among other alphaherpesviruses.