The monoclonal antibody anti-M116, clone M116.02, recognizes MCMV protein M116.1p.
The MCMV M116 gene locus encodes two late, 5′-coterminal, spliced, and polyadenylated transcripts: M116-M114 and M116, both encoding the M116.1 protein (Ruzic, Juranic Lisnic et al, J Virol, 2021). Protein M116.1 is conserved among different strains of MCMV, N-glycosylated, expressed with late kinetics and localized to the outer ring of the cytoplasmic virion assembly compartment where it interacts with gH, potentially acting as its chaperone in a similar fashion to homologous HCMV protein UL116. Although protein M116.1 is necessary for efficient infection of mononuclear phagocytes in vitro, its deficiency has no significant effect on virus spread during systemic or footpad infection. However, reduced ability of ΔM116-MCMV to spread successfully upon intranasal infection is observed.
Our anti-M116 antibody clone M116.02 had been validated in Western blot (MCMV infected cell lysates and recombinant protein), flow cytometry (MCMV infected cells), immunohistochemistry (livers of MCMV-infected BALB/c mice), immunofluorescence (MCMV infected cells), co-immunoprecipitation (MCMV infected cells) and proximity ligation assay (MCMV infected cells).