The monoclonal antibody m04.16 recognizes extracellular domain of MCMV protein m04.
m04 (also known as gp34) is a 34-kDa type I glycoprotein, member of MCMV m02-m16 family that plays a role in major histocompatibility complex class I (MHC I) trafficking and immune evasion. m04 protein is abundantly expressed in the endoplasmic reticulum (ER) with early kinetics of expression, starting at 4 hours post infection (EMBO J 1997. 16:685-94.). m04, together with MCMV protein MATp1, binds to MHC I in the ER and escorts some proportion of MHC I to the cell surface where they engage inhibitory Ly49 receptors in order to prevent NK cell activation and killing (J Exp Med 2010. 207:2663-73.; J Exp Med 2019. 216(8):1809-1827.). It is one of the most abundantly produced MCMV proteins with great number of polymorphic variants identified in MCMV field isolates (Virology 2006. 352: 450-65.)
Our anti-m04 antibody has been validated in ELISA (on immunogen), IF (MCMV infected cells), IP (MCMV infected cell lysates), FACS (MCMV infected cells), IHC on PFFE (tissues from infected mice) and proximity ligation assay (infected cells). It has been shown to block interactions between protein m04 and Ly49 receptors on the surface of infected cells, while still enabling Ly49A-MHC I interaction as described in J Exp Med 2019. 216(8):1809-1827.