The monoclonal antibody m04.17 recognizes extracellular domain of MCMV protein m04.
m04 (also known as gp34) is a 34-kDa type I glycoprotein, member of MCMV m02-m16 family that plays a role in major histocompatibility complex class I (MHC I) trafficking and immune evasion. m04 protein is abundantly expressed in the endoplasmic reticulum (ER) with early kinetics of expression, starting at 4 hours post infection (EMBO J 1997. 16:685-94.). m04, together with MCMV protein MATp1, binds to MHC I in the ER and escorts some proportion of MHC I to the cell surface where they engage inhibitory Ly49 receptors in order to prevent NK cell activation and killing (J Exp Med 2010. 207:2663-73.; J Exp Med 2019. 216(8):1809-1827.). It is one of the most abundantly produced MCMV proteins with great number of polymorphic variants identified in MCMV field isolates (Virology 2006. 352: 450-65.)
Our anti-m04 antibody has been validated in ELISA (on immunogen), IF (MCMV infected cells), IP (MCMV infected cell lysates) and FACS (MCMV infected cells) as described in J Exp Med 2019. 216(8):1809-1827.