Recombinant SARS-CoV-2 Nucleocapsid protein (His tag)
Species: SARS-CoV-2
Expression System: prokaryotic
Amino acid sequence: Met1-Ala419
Tag: C-terminal 6-His tag
Predicted molecular weight: 47 kDa
Type: recombinant protein
Conjugate: unconjugated
Recombinant SARS-CoV-2 Nucleocapsid protein (His tag)
SDS-PAGE with Coomassie Blue (left) and Western blot with anti-His Ab (right) of SARS-COV-2 Nucleocapsid (aa1-419), His Tag Recombinant Protein showing a dominant band at 55 kDa.
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Description
SARS-CoV-2 Nucleocapsid protein (nucleoprotein, N protein) is one of the four major structural proteins of SARS-CoV-2 virus, together with membrane (M), envelope (E) and Spike (S) protein. It is involved in transcription, replication and packaging of the viral RNA genome into a helical ribonucleocapsid.
The SARS-CoV-2 N protein is a ~45 kDa protein and one of the most abundantly expressed viral proteins. Its amino acid sequence is largely conserved and it shares 91% and 47% amino acid sequence identity with SARS-CoV-1 and MERS N protein, respectively. It displays high immunogenic activity which makes it a suitable candidate for detection of patient anti-N antibodies and use in serological diagnostic kits.
Additional information
| FORM | Liquid |
|---|---|
| STORAGE | -20°C. Avoid freeze-thaw cycles. |
| STORAGE BUFFER | DEB buffer (8M urea, 200mM Na3PO4, 500mM NaCl; pH 4.0) |
| PURITY | Affinity purified |
| REFERENCES | Pribanić Matešić et al. Viruses 2022, 14(2), 443. |
