The monoclonal antibody UL11.01 recognizes HCMV protein pUL11.
pUL11 is a type I transmembrane glycoprotein encoded by the human cytomegalovirus (HCMV) UL11 gene. This gene belongs to the CMV RL11 family, which includes genes that are non-essential for viral replication but contribute to virulence (Arch Virol. 1997;142(7):1407-27). pUL11 has been shown to interact specifically with the receptor tyrosine phosphatase CD45 on T cells. By binding to CD45, pUL11 inhibits TCR-mediated signaling, reducing tyrosine phosphorylation of signaling proteins and impairing T cell proliferation. The UL11 open reading frame (ORF) generates proteins of varying sizes (in WB a 50 kDa band and several bands spanning 60-72 kDa size can be observed). This is determined by post-translational modifications and the position of the translation start site. Research indicates that the highly glycosylated UL11 forms are located on the cell surface, whereas the less glycosylated forms are restricted to the endoplasmic reticulum (PLoS Pathog. 2011 Dec 8;7(12):e1002432 and J Virol. 2014 Dec;88(24):14326–14339).