Anti-Spike (SARS-CoV-2) antibody – clone S1.01
Clone: S1.01
Catalog No.: HR-SARSCov2-5
Host Species: Mouse
Reactivity: SARS-CoV-2
Antigen/Immunogen: HEK293T produced His-tagged RBD
Tested Applications: ELISA, FACS, IF
€200.00 – €1,500.00
Anti-Spike (SARS-CoV-2) antibody - clone S1.01
Overlay histogram showing WT (Wuhan) SARS-CoV-2 infected Vero E6 cells stained with mouse anti-S1.01 (red line) and FITC-conjugated anti-mouse IgG. Mock infected cells (grey line) were used under the same conditions.
Anti-Spike (SARS-CoV-2) antibody - clone S1.01
Immunofluorescence: SARS-CoV-2 infected Vero E6 cells were stained with S1.01 antibody followed by TRITC-coupled anti-mouse antibodies (red) and nuclei staining by DAPI (blue).
Anti-Spike (SARS-CoV-2) antibody - clone S1.01
Overlay histograms showing 293T-ACE2 cells infected with indicated SARS-CoV-2 variants and stained with mouse anti-S1.01 (red line) and Alexa fluor 647-conjugated anti-mouse IgG. Mock infected cells (grey line) were used under the same conditions.
Anti-Spike (SARS-CoV-2) antibody - clone S1.01
Immunofluorescence: SARS-CoV-2 Delta variant infected Vero E6 cells were stained with S1.01 antibody followed by TRITC-coupled anti-mouse antibodies (red) and nuclei staining by DAPI (blue).
Anti-Spike (SARS-CoV-2) antibody - clone S1.01
Immunofluorescence: SARS-CoV-2 infected Vero E6 cells were stained with S1.01 antibody followed by TRITC-coupled anti-mouse antibodies (red) and nuclei staining by DAPI (blue).
Anti-Spike (SARS-CoV-2) antibody - clone S1.01
Overlay histograms showing 293T-ACE2 cells infected with indicated SARS-CoV-2 variants and stained with mouse anti-S1.01 (red line) and Alexa fluor 647-conjugated anti-mouse IgG. Mock infected cells (grey line) were used under the same conditions.
Anti-Spike (SARS-CoV-2) antibody - clone S1.01
Immunofluorescence: SARS-CoV-2 Delta variant infected Vero E6 cells were stained with S1.01 antibody followed by TRITC-coupled anti-mouse antibodies (red) and nuclei staining by DAPI (blue).
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Description
The monoclonal antibody S1.01 recognizes SARS-CoV-2 Spike protein.
SARS-CoV-2 Spike protein is a structural transmembrane glycoprotein comprised of two subunits (S1 and S2), size of 180–200 kDa. It protrudes from the surface of the SARS-CoV-2 virion and is essential for binding to the host cell via host ACE-2 receptor. Spike protein is a main target for neutralizing antibodies and vaccines. Our anti-Spike antibody clone S1.01 was validated in ELISA, FACS (on SARS-CoV-2 infected 293T-ACE2 cells and Vero E6 cells) and IF (on SARS-CoV-2 infected Vero E6 cells) using WT virus and several variants of concern, as described in Pribanić Matešić et al. Viruses 2022, 14(2), 443. The S1.01 antibody does not perform in WB.
Additional information
| FORM | Liquid |
|---|---|
| STORAGE | Long term -20 °C, short term +4 °C. Avoid freeze-thaw cycles. |
| CONCENTRATION | 1 mg/mL |
| PURITY | Affinity purified |
| CLONALITY | Monoclonal |
| ISOTYPE | IgG1 |
| LIGHT CHAIN TYPE | kappa |
| REFERENCES | Pribanić Matešić et al. Viruses 2022, 14(2), 443. |
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