The monoclonal antibody VZ 9C.09 recognizes VZV ORF9.
During VZV infection, ORF9 is the most transcribed VZV gene (J Virol 2003. 77: 11718-11732., J Gen Virol 2005. 86: 2673-2684.). ORF9 protein is a 302 aminoacids long tegument protein expressed with late kinetics. It is a member of herpesvirus UL49 gene family (J Gen Virol 1986. 67 (Pt 9): 1759-1816). It has an essential role in VZV replication (J Virol 2007. 81: 13200-13208) and a role in secondary envelopment (J. Virol. 87: 2868-2881). The protein is localized in the cytoplasm and has a reported role in the recruitment of IE62 to complexes within the cytoplasm (J Virol 2007. 81: 761-774).
Our anti-ORF9 antibody has been validated in ELISA (on immunogen), IF (VZV infected cells) and WB (VZV infected cell lysates) as described in J Virol. 2013 Jun;87(12):6943-54.