The monoclonal antibody VZ 7.05 recognizes VZV ORF7.
VZV ORF7 (UniProtKB – Q6QCP9) is the ortholog of HSV-1 protein UL51. Zhang et al (PLoS Pathog. 2010 Jul 1;6:e1000971) showed that ORF7 is required for viral replication in skin organ cultures (a skin tropism factor) and Selariu et al (J Virol. 2012 Aug;86(16):8614-24. doi: 10.1128) showed how ORF7 is a neurotropic factor, where its deletion completely abolishes viral spread in human nervous tissue. Our monoclonal antibody to ORF7, reveals a protein with dominant localisation in Golgi networks and a dominant band around 35 kDa in Western blot.
Our anti-ORF7 has been validated in ELISA (on immunogen), IF (VZV infected cells) and WB (VZV infected cell lysates) as described in J Virol. 2013 Jun;87(12):6943-54.