ORF47 protein is a tegument protein which possesses a kinase activity (J Gen Virol 1994. 75 (Pt 2): 317-326, Virology 1992. 191: 9-18) and is a homolog of herpes simplex type 1 (HSV-1) UL13, human cytomegalovirus (hCMV) UL97 and Epstein Barr virus (EBV) BFLF4 protein kinases (J Virol 1989. 63: 450-455). This protein is essential for the T cell tropism (Proc Natl Acad Sci U S A 1998. 95: 11969-11974) and for VZV replication in immature dendritic cells (DC), but not in mature DC (Virology 2005. 337: 304-311). The ORF47 kinase was reported to form a complex with VZV IE62 (J Virol 2003. 77: 5964-5974). Our anti-VZV47 antibody VZ 47.01 works in ELISA (on immunogen), IF (on VZV infected cells) and each LOT is validated for Western blot of VZV infected cell lysates, where it gives a pattern as described in J Virol. 2013 Jun;87(12):6943-54.).
Clone: VZ 47.01
Catalog No.: HR-VZV-31
Host Species: Mouse
Reactivity: Varicella zoster virus
Antigen/Immunogen: The immunogen consisted of baculovirus-derived full length ORF47 protein
Tested Applications: ELISA; WB
Recommended Dilution: n/a
Varicella zoster virus
The immunogen consisted of baculovirus-derived full length ORF47 protein
€200.00 – €700.00
|STORAGE||Long term -20 °C, short term +4 °C. Avoid freeze-thaw cycles.|
|LIGHT CHAIN TYPE||kappa|
|REFERENCES||Lenac et al., J Virol, 2013|
VZV ORF33.5 encodes a scaffold protein involved in the assembly of the viral capsid. ORF33.5 has the capacity to mediate ORF40 translocation into the nucleus of VZV-infected cells and exhibits a nuclear localization phenotype.
VZV ORF23 encodes a 24 kDa small capsid surface protein that localizes primarily to cell nuclei during infection and is not essential for viral replication in cell culture, even though its absence disrupts the capsid assembly.
VZV ORF4 encodes an IE4 protein that acts as transactivator of several immediate early, early and late VZV genes. Dimerization of IE4 is required for its transactivation function.