The ORF32 encodes a protein of 16 kDa located in the cytosol of virus-infected cells and post – translationally modified by the ORF47 protein kinase. Since the VZV ORF47 protein kinase has recently been shown to be critical for virus replication in human fetal skin and lymphocytes, its ability to modify the ORF32 protein suggests that the latter protein may have a role for VZV replication in human tissues. Our anti-VZV ORF32 antibody VZ 32.01 (described in J Virol. 2013 Jun;87(12):6943-54.) works in ELISA (on immunogen) and IF (on VZV infected cells) and each LOT is validated for Western blot (of VZV infected cell lysates) where it specifically recognizes a protein species of ~16 kDa in lysates obtained from VZV infected cells.