The monoclonal antibody VZ 1N.03 recognizes VZV ORF1.
The ORF1 protein is one of 5 VZV unique genes that are not present in HSV-1. Cohen et al (Virology. 1995 Feb 1;206(2):835-42) analysed ORF1 by using antibody against epitope inserted in the ORF1 of recombinant virus. The ORF1 protein they detected in the membrane of infected cells was slightly larger than predicted suggesting post-translational modification. Koshizuka et al (Virology 2008. 377:289 –295) described ORF1 gene product as a tail-anchored membrane protein localized to plasma membrane and trans-Golgi network in infected cells. Our VZ 1N.03 antibody confirmed that ORF1 localizes in the Golgi network and other membranes in VZV infected cells and also confirmed that ORF1 is slightly larger (16–20 kDa) than predicted (12.1 kDa).
Our anti-ORF1 antibody has been validated in ELISA (on immunogen), IF (VZV infected cells) and in WB (VZV infected cell lysates) as described in J Virol. 2013 Jun;87(12):6943-54.