VZV contains 5 unique genes (ORF1, ORF2, ORF13, ORF32, and ORF57) not present in herpes simplex virus 1 (HSV-1). Cohen et al (Virology. 1995 Feb 1;206(2):835-42) analysed ORF1 by the antibody to the epitope inserted in the ORF1 of recombinant virus. The ORF1 protein they detected in the membrane of infected cells was slightly larger suggesting post-translational modification in infected cells. Koshizuka et al (Virology 2008. 377:289 –295) described ORF1 gene product as a tail-anchored membrane protein localized to plasma membrane and trans-Golgi network in infected cells. Only rabbit sera was available to analyse ORF1 (UniProtKB – Q4JQX4) before we generated VZV mAbeom. The VZ 1N.03 antibody confirmed in IF that ORF1 is localized to the Golgi network and other membranes in VZV infected cells. The ORF1 has calculated molecular weight of 12.1 kDa and our VZ 1N.03 confirmed slightly bigger protein of 16–20 kDa in Western blot. Our anti-VZV ORF1 antibody VZ 1N.03 works in ELISA (on immunogen), IF (on VZV infected cells) and each LOT is validated for Western blot (of VZV infected cell lysates, described in J Virol. 2013 Jun;87(12):6943-54.).
Clone: VZ 1N.03
Catalog No.: HR-VZV-39
Host Species: Mouse
Reactivity: Varicella zoster virus
Antigen/Immunogen: The immunogen consisted of ORF1 N terminal domain (amino acids: 1-73) and was produced in E.coli
Tested Applications: ELISA; IF; WB
€120.00 – €500.00