The monoclonal antibody N.01 recognizes SARS-CoV-2 nucleocapsid protein, also known as nucleoprotein or N protein.  

Nucleocapsid protein is one of the four major structural proteins of SARS-CoV-2 virus, together with membrane (M), envelope (E) and Spike (S) protein. It is involved in transcription, replication and packaging of the viral RNA genome into a helical ribonucleocapsid. The SARS-CoV-2 N protein is a ~45 kDa protein and one of the most abundantly expressed viral proteins.  Its amino acid sequence is largely conserved and it shares 91% and 47% amino acid sequence identity with SARS-CoV-1 and MERS N protein, respectively. It displays high immunogenic activity which makes it a suitable candidate for detection of patient anti-N antibodies and use in serological diagnostic kits.  

Our anti-nucleocapsid antibody N.01 has been validated in ELISA (on recombinant Nucleocapsid protein), western blot (recombinant Nucleocapsid protein and lysates of SARS-CoV-2 infected Vero cells), in IF and FACS (SARS-CoV-2 infected Vero cells), as described in Pribanić Matešić et al. Viruses 2022, 14(2), 443). For more details on the immunogen, please see our product cat.nr. NP-100. 

FORM	Liquid
STORAGE	Long term -20 °C, short term +4 °C. Avoid freeze-thaw cycles.
CONCENTRATION	1 mg/mL
PURITY	Affinity purified
CLONALITY	Monoclonal
ISOTYPE	IgG1
LIGHT CHAIN TYPE	kappa
REFERENCES	Pribanić Matešić et al. Viruses 2022, 14(2), 443.

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