The monoclonal antibody N.01 recognizes SARS-CoV-2 nucleocapsid protein, also known as nucleoprotein or N protein.
Nucleocapsid protein is one of the four major structural proteins of SARS-CoV-2 virus, together with membrane (M), envelope (E) and Spike (S) protein. It is involved in transcription, replication and packaging of the viral RNA genome into a helical ribonucleocapsid. The SARS-CoV-2 N protein is a ~45 kDa protein and one of the most abundantly expressed viral proteins. Its amino acid sequence is largely conserved and it shares 91% and 47% amino acid sequence identity with SARS-CoV-1 and MERS N protein, respectively. It displays high immunogenic activity which makes it a suitable candidate for detection of patient anti-N antibodies and use in serological diagnostic kits.
Our anti-nucleocapsid antibody N.01 has been validated in ELISA (on recombinant Nucleocapsid protein), western blot (recombinant Nucleocapsid protein and lysates of SARS-CoV-2 infected Vero cells), in IF and FACS (SARS-CoV-2 infected Vero cells), as described in Pribanić Matešić et al. Viruses 2022, 14(2), 443). For more details on the immunogen, please see our product cat.nr. NP-100.