The monoclonal antibody 1D6 recognises mouse MULT-1.
MULT-1 (murine UL-16 binding protein-like transcript 1) is one of the cellular stress-induced ligands for mouse NKG2D receptor. MULT-1 was discovered by the sequence homology with human UL-16 binding proteins (ULBPs). It has the highest affinity for the receptor among all known mouse NKG2D ligands (Kd = 6 nM) (Eur J Immunol 2003.33(2):381-91; J Immunol 2002. 169(8):4079-83.) Its transcripts were found in most of the healthy tissues, but its protein expression was strictly regulated on posttranslational level (J Exp Med 2009. 206(2):287–298.). It is a type I transmembrane glycoprotein with long cytoplasmic tail and protein sequence of 334 amino acids. Estimated molecular weight is 53 kDa and half-life of around 16 hours (J Exp Med 2005. 201(2):211-20.)
Our anti-MULT-1 antibody has beed validated in ELISA (on cell lysates), in IP (on MULT-1-HA transfectants) and in flow cytometry on several different cell lines as described in references.