Our mAbs clones CROMA 7, m55.01 and m55.02 recognize MCMV protein m55, also known as gB. gB is a major envelope transmembrane glycoprotein, part of the protein machinery promoting the fusion process involved in virus entry. It is encoded by a gene transcribed in late phase of infection (after 16 h p.i.). gB is highly conserved among different herpesviruses and is a major target of virus-neutralizing antibody responses in CMV-infected animals and humans (Britt WY et al, J Virol, 1990). In addition, HCMV gB is also involved in cell adhesion and signaling capabilities (Boyle, KA et al, J Virol, 1998; Boyle, ka et al, Mol Cell Biol. 1999. gB is present in several protein isoforms of different molecular masses, with the 130kDa form being gB precursor protein (Rapp, M et al, J Virol, 1992). It has been proposed for HCMV that a cellular protease cleaves precursor gB resulting in additional protein forms of lower molecular mass (Spaete RR et al, Virology 1988). This can be also observed by staining MCMV-infected cells with our M55.01 clone in western blot since this clone recognizes all forms. On the other hand, cloneM55.02 recognizes only the form of the highest molecular weight (130kDa). Additional tested applicatons are as follows: Croma 7 (described in the Journal of Infectious Diseases (2003) 187 (6): 988-999) works in IHC; M55.01 and M55.02 work in flow cytometry; M55.01 and Croma7 work in IF. Each LOT is validated for western blot on MCMV infected cell lysates.
Clone: CROMA 7
Catalog No.: HR-MCMV-04
Host Species: Mouse
Reactivity: Murine cytomegalovirus
Antigen/Immunogen: The immunogen consisted of MCMV virions
Tested Applications: IHC
The immunogen consisted of MCMV virions
€120.00 – €500.00
|STORAGE||Long term -20 °C, short term +4 °C. Avoid freeze-thaw cycles.|
|LIGHT CHAIN TYPE||n/a|
|REFERENCES||The Journal of Infectious Diseases (2003) 187 (6): 988-999|
Murine cytomegalovirus (MCMV) M57 gene region encodes for a 131-kDa M57 protein considered the MCMV major single-stranded DNA binding protein (Kattenhorn et al., 2004, Journal of Virology, 78(20), 11187–97).
Murine cytomegalovirus immediate-early 1 (IE1, pp89, m123) is one of the first proteins expressed during productive (lytic) infection as well as during reactivation from the latency.
MCMV early protein 1 (E1), encoded by murine cytomegalovirus (MCMV) M112-113 gene region localizes predominately to the nucleus of MCMV-infected cells (Ciocco-Schmitt et al., 2002, Virology, 294, 199–208.).
M45 is a 97-kDa virion-associated protein encoded by mouse cytomegalovirus (MCMV) (Krause et al., 2014, Journal of Virology, 88(17), 9963–9975; Rawlinson et al., 1996, Journal of Virology, 70(10), 8833–8849).
Our mAbs clones CROMA 7, m55.01 and m55.02 recognize MCMV protein m55, also known as gB. gB is a major envelope transmembrane glycoprotein, part of the protein machinery promoting the fusion process involved in virus entry.