Murine cytomegalovirus immediate-early 1 (IE1, pp89, m123) is one of the first proteins expressed during productive (lytic) infection as well as during reactivation from the latency. In addition to serving as regulatory co-activator transcription of viral genes, IE1 also has multiple roles in priming the cell for efficient viral gene expression (e.g. by disrupting PML or ND10 bodies or reducing histone deacetylase activity. IE1 an IE3 proteins are translated from alternatively spliced transcripts from the same locus: IE1 contains exons 1 to 4 while IE3 contains exons 1-3 and also contains exon 5. IE1 protein is 89 kDa in size. Our anti-m123/IE1 antibodies IE1.01 and Croma101 (described in Journal of General Virology (2005), 86, 2153-2161; Journal of Virology (2009), 83 (3): 1260-1270; Microbes and Infection (2003), 13 (5) 1263-1277; Journal of General Virology (2005), 86, 2153-2161; Journal of General Virology (1998), 79, 867-876) work in IF (on MCMV infected cells), immunohistology/IHC (on paraffin sections) and each LOT is validated for Western blot (of MCMV infected cell lysates).
Catalog No.: HR-MCMV-08
Host Species: Mouse
Reactivity: Murine cytomegalovirus
Antigen/Immunogen: The immunogen consisted of MCMV virions
Tested Applications: IF; IHC; WB
The immunogen consisted of MCMV virions
IF; IHC; WB
€120.00 – €500.00
|STORAGE||Long term -20 °C, short term +4 °C. Avoid freeze-thaw cycles.|
|LIGHT CHAIN TYPE||kappa|
|REFERENCES||Journal of General Virology (1998), 79, 867-876;
Journal of General Virology (2005), 86, 2153-2161;
Journal of Virology (2009), 83 (3): 1260-1270;
Microbes and Infection (2003), 13 (5) 1263-1277
MCMV early protein 1 (E1), encoded by murine cytomegalovirus (MCMV) M112-113 gene region localizes predominately to the nucleus of MCMV-infected cells (Ciocco-Schmitt et al., 2002, Virology, 294, 199–208.).
m155 is a mouse cytomegalovirus (MCMV) 43-kDa glycoprotein expressed in endoplasmic reticulum and cis-Golgi compartment of infected cells (Loewendorf et al., 2011, Journal of Virology, 85(10), 5208–5212).
Murine cytomegalovirus immediate-early 1 (IE1, pp89, m123) is one of the first proteins expressed during productive (lytic) infection as well as during reactivation from the latency.
M45 is a 97-kDa virion-associated protein encoded by mouse cytomegalovirus (MCMV) (Krause et al., 2014, Journal of Virology, 88(17), 9963–9975; Rawlinson et al., 1996, Journal of Virology, 70(10), 8833–8849).
Murine cytomegalovirus (MCMV) M57 gene region encodes for a 131-kDa M57 protein considered the MCMV major single-stranded DNA binding protein (Kattenhorn et al., 2004, Journal of Virology, 78(20), 11187–97).
Our mAbs clones CROMA 7, m55.01 and m55.02 recognize MCMV protein m55, also known as gB. gB is a major envelope transmembrane glycoprotein, part of the protein machinery promoting the fusion process involved in virus entry.