m06, also known as gp48, is a member of MCMV m04-m16 family playing a role in MHC-I trafficking and immune evasion. m06 is a 48-kDa type I transmembrane glycoprotein with early kinetics of expression (3h), peaking at 3–6 h p.i. (Reusch, U. et al, The EMBO Journal. 1999). Unlike m04, this glycoprotein binds stably to MHC-I complexes in the ER and moves with them via the Golgi apparatus to the trans-Golgi network redirecting MHC I complexes to lysosomes for degradation, thus preventing their surface expression. Our anti-m06 antibody CROMA 229 (described e.g. in The EMBO Journal (1999) 18, 1081-1091; Nature Immunology (2002) 3, 529-535; Journal of Experimental Medicine (2009) 206, 515-523) works in WB (on MCMV infected cell lysates) and each LOT is validated for intracellular FACS (on MCMV infected cells).